Asparagine Biosynthesis in Soybean Nodules
نویسندگان
چکیده
منابع مشابه
Asparagine biosynthesis in soybean nodules.
Asparagine biosynthesis in soybean (Glycine max [L.] Merr.) nodules has been difficult to demonstrate due to the poor conversion of suspected immediate precursors to asparagine and the instability of the key enzyme asparagine synthetase. The present study was designed to explore the effects of two ammonium assimilation inhibitors on the metabolism of (14)CO(2) to [(14)C]asparagine and to demons...
متن کاملAsparagine formation in soybean nodules.
(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glu...
متن کاملPropionate in heme biosynthesis in soybean nodules.
When soybean nodules are incubated with propionate-2-(14)C the heme moiety of leghemoglobin becomes labeled. The incorporation of propionate-2-(14)C into heme is linear with time and it appears that propionate is utilized without a lag period. The rate of incorporation of propionate-2-(14)C into heme is more rapid than the rate of incorporation of succinate-2-(14)C and citrate-1,5-(14)C, howeve...
متن کاملAsparaginase and asparagine transaminase in soybean leaves and root nodules.
Asparaginase activity (</=1 mumol/mg protein . hr) was detected in extracts of soybean (Glycine max [L.] Merr.) leaf blades, but, even after efforts to optimize extraction and assay of the enzyme, specific activity was not sufficient to metabolize the estimated amount of asparagine translocated to leaves. Asparagine transaminase activity with glyoxylate or pyruvate was at least 52 and 62 nmol/m...
متن کاملEnzymes of ammonia assimilation and ureide biosynthesis in soybean nodules: effect of nitrate.
The effect of nitrate on N(2) fixation and the assimilation of fixed N(2) in legume nodules was investigated by supplying nitrate to well established soybean (Glycine max L. Merr. cv Bragg)-Rhizobium japonicum (strain 3I1b110) symbioses. Three different techniques, acetylene reduction, (15)N(2) fixation and relative abundance of ureides ([ureides/(ureides + nitrate + alpha-amino nitrogen)] x 10...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1984
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.74.3.605